Studi e ricerche scientifiche sulle qualità dell’acqua Kaqun.

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2016 – VIII Congresso Internazionale ARTOI

arti kaqun ODUEIn occasione dell’ VIII Congresso Internazionale ARTOI tenutosi a Firenze l’11, il 12 e il 13 novembre 2016, la Dott.ssa Michela Marinato ha presentato un poster relativo allo studio condotto dal Dott. Janos Hunyadi – membro della Commissione Scientifica KAQUN Europa.

INVESTIGATION OF ELECTROLYSED (KAQUN) WATER ON GROW OF HUMAN OVARIAN CARCINOMA AND HUMAN CERVIX CARCINOMA XENOGRAFTS IN SCID MICE

Dr. Janos Hunyadi MD

Department Debrecen, Hungary. National Institute of Oncology, Budapest, Hungary Presenting author: Dr. Marinato Michela michela@strudiomarinato.it

Introduction
In the present study we investigated the influence of KAQUN water consumption on the in vivo intake, and growth-kinetic of human tumor cells in xenotransplants grafted to mice with Severe
Combined Immune-deficient (SCID).
Materials and Methods: SCID mice allow routine engraftment and growth of xenogenic cells due to their abnormal humoral and cellular immune defense mechanism. Human ovarian carcinoma cells (A2780), and cells derived from human cervix carcinoma (KB-3-1) were transplanted to SCID mice in 4 x 106 and in 2 x 106 cell numbers respectively. Control mice were drinking normal water (n=9), while others received the oxygen enriched Kaqun water (n=9). One further group of animals (n=5) started to drink Kaqun water 14 days before xenotransplantation. Volume of growing xenografts was estimated 11, 17, 25 and 33 days after xenotransplantation. One mouse was sacrificed in each investigated groups on days 18 and 26 and all others on days 33 for obtaining tumors and internal organs for histological investigation.
Results: Seventeen days after xenotransplantation the volume of tumor were significantly smaller in Kaqun and pre-Kaqun group in the case of both type of tumors. We suppose that:
a./ the lower death rate
b./ the decreased tumor take
c./ the decreased growth kinetic in Kaqun water consuming groups and
d./ the tendency of tumor-regression observed at 5 of the Kaqun water treated mice might be the consequence of the changed tissue oxigenisation. Histological investigation of the growing tumors stated that Kaqun resulted in a high apoptotic activity and damaged tumor cells, which correlates with the clinical data.
Conclusion: The studies about Kaqun water began 20 years ago in USA and have been perfected in Hungary. This particular water is produced through a sophisticated technological process of electrolysis: it is the only one in the world which contains bioavailable and stable oxygen (long term) in an amount from 15 up to 26 mg / liter. It is alkaline with a pH medium value from 7.8 to 8.2. It remains stable for over one year under standard conditions and it is able to increase oxygenation level of body tissues preventing Warburg effect.

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kaqun

Using KAQUN in sport can be an important factor in optimizing performance:

  • Sufficient amount varies according to branches of sport
  • Determination of personal application is essential
  • Higher tissue oxygen levels provide quicker regeneration and better loadability
  • Simultaneous load in the foundation period is more tolerable (if performed with proper training methods), therefore the quality of training can improve

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2012 – Therapeutic effect by a mistletoe extract, Iscador and oxygen enriched water (Kaqun) on the development of experimental …

Universitas Debreceniensis Debrecen, Hungary

studies 3Kaqun is a special water with high oxygen content, suitable for use in the form of drinking water or bathing. As a result of the KAQUN technology oxygen is present in a stable form with a concentration of 18-25 mg/l, which is manifold of the oxygen content of normal drinking water. Killing of cancer cells by macrophages in a non-ADCC manner via apoptosis is induced by reactive oxygen species (ROS). (Hicks AM, Willingham MC, Du W, Pang CS, Old LJ, Cui Z: Effector mechanisms of the anti-cancer immune responses of macrophages in SR/CR mice. Cancer Immun. 2006 Oct 31;6:11). It was also observed, that target cell antioxidant mechanisms play an important role in the outcome of the cytotoxic response of human polymorphonuclear leukocytes (PMN) against red blood cells (RBC) and K562 tumor cells (van Kessel KP, van Strijp JA, van Kats-Renaud HJ, Miltenburg LA, Fluit AC, Verhoef J.: Uncoupling of oxidative and non-oxidative mechanisms in human granulocyte-mediated cytotoxicity: use of cytoplasts and cells from chronic granulomatous disease patient. J Leukoc Biol. 1990 Oct;48(4):359-66). Kaqun water has a significant influence on grow of tumor cells in vitro, although there are great differences between its effect on different cell lines. It has no influence on Hep G2 (human hepatocellular carcinoma) cells (02-ctox-10, OKBI-KBKF Budapest, 2010.12.20), while it significantly decreases the growth of LLT-HH (highly metastatic variant of Lewis lung carcinoma) and H59 (Lewis Lung Carcinoma Variant) cell lines in a dose dependent manner (). Furthermore human experiences suggest a benefit effect of kanqun water on several human tumors (including ovarian carcinoma)

2012 – Report about effects of Kaqun water on the speed of cognitive functions – Permit Number: IV-R-015-14-4/2012

SOUTH BORSOD HEALTH CARE SOCIAL CLUSTER Miksolc, Hungary

studies 1Our research project: The effects of Kaqun water on the speed of cognitive functions” was conducted on randomly selected healthy elderly individuals who fit the age group. The average age of the groups was around 65 years. We made 4 groups, 1.5l; 1l; 0.5l daily consumption of Kaqun water as well as the control group whose members drank 1l tap water. During the measurements the people who made the measurements and who assessed the results did not know who was in which group, it was only made known to them after the data have been processed (double blind). The connection between the dose and the therapeutic effect has also been examined.

We examined: plethysmogram, the spread of the pulse relaxed and under load with tools of HRV analysis; blood pressure (systolic and diastolic), oxygen saturation, SRT (reaction time) and CRT (cognitive reflex time).

The results of the measurements and the significance of the changes were assessed by RopStat software.

 

2011 – Study for the KAQUN’s effect for antioxydant capacity (TUKEB No.: 550/2011)

NATIONAL INSTITUTE OF CHEMICAL SAFETY –  DEPARTMENT OF MOLECULAR AND CELL BIOLOGY, Budapest, Hungary

studies 2Aim of the study
Our aim was to study the effect of a regimen of bathing and drinking Kaqun water on the antioxidant capacity of healthy volunteers, to establish whether the treatment changes the antioxidant parameter compared to the value before treatment, and whether the gender of the subject affects the measured parameters. The studied parameters were analysed at individual and group level. The total antioxidant capacity of serum and erythrocyte lysate obtained from whole blood was evaluated, compared to the 0 point, initial values.

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2010 – Citotoxicity examination of Kaqun water in HepG2 cells /NICS/

NATIONAL INSTITUTE OF CHEMICAL SAFETY –  DEPARTMENT OF MOLECULAR AND CELL BIOLOGY, Budapest, Hungary

studies 2PRINCIPAL INVESTIGATOR’S DECLARATION
I the undersigned hereby declare that the toxicity examination titled Citotoxicity examination of Kaqun water in HepG2 cells (with examination number: 02-CTOX-10) was carried out in compliance with the regulations of OECD Principles of Good Laboratory Practice (ENV/MC/CHEM(98)17) at the Molecular and Cell Biological Department of the Department of Research for Chemical Safety of the National Institute of Chemical Safety (OKBI).

The examination was carried out based on the decrees titled Biological evaluation of medical devices Part 5: Tests for citotoxicity: in vitro methods (ISO 10993-5: 1992), MSZ EN 30993-5:1998; Biological evaluation of medical devices Part 12: Sample preparation and reference materials (ISO 10993-12: 2007), MSZ EN 10993-12:2008.

The examination was carried out according to Standard Operations Regulations of OKBIKBKF-MSBO.
The Closing Report is based on correct examination data and the obtained results are in compliance with the content of the Closing Report.

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2010 – Report on the examination of KAQUN oxygen-rich water’s role in reactive oxygen species generation in in vitro system /HAS/

NATIONAL INSTITUTE OF CHEMICAL SAFETY –  DEPARTMENT OF MOLECULAR AND CELL BIOLOGY, Budapest, Hungary

studies 2The principle of the method
Horse radish peroxidase produces reactive oxygen species from peroxide. This converts benzidine into a colourful product with kinoid structure. The change of colour concentration may be photometrically measured at 620 nm. Thus the system – being a highly sensitive procedure – is suitable for detecting reactive oxygen species. By the use of this method it can be assessed whether oxygen-rich water increases the quantity of reactive oxygen species in the system or not. Peroxidase first reduces molecular oxygen solved in the oxygen-rich water to peroxide, then thus produces a greater quantity of reactive oxygen species from the increased peroxide in the system.

This method may also be used for measuring antioxidant capacity. The presence of an antioxidant, e.g. ascorbic acid, poliphenol, or uric acid inhibits the formation of the colour.

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2009 – The effect of KAQUN water on the immune parameters of healthy volunteers (TUKEB No.: 42/2009)

NATIONAL INSTITUTE OF CHEMICAL SAFETY – CITOGENETIC AND IMMUNOLOGIC DIVISION, Budapest, Hungary

studies 2Summary

  1. No biologically significant changes were observed in the qualitative and quantitative blood count either at group level or individual level during the 21 days of Kaqun treatment.
  2. The percentage of NK-cells showed a statistically significant increase, and the individual changes (increase) relative to the 0 point were bigger, which may have a functional impact, namely that more NK cells are available to kill virus infected and cancerous cells.
  3. A non-specific activation of T lymphocytes (indicated by the increase in the expression of the CD25 cell surface antigen) could be detected, presumably caused by the Kaqun treatment, indicating the increased activity of the cellular immune response.
  4. Characteristically the value of several parameters changed significantly by the second week of treatment and during the third week the value of the parameter remained at the same level, or the change levelled to its original value (percent of neurophils, monocytes, activated (CD25+) T cells, activated (CD25+) helper T cells and CD71+ B cells). This suggests that two weeks treatment is the most effective for the change in immune parameters and after that the reaction of the body to the treatment decreases, that is, the effect cannot be boosted.
  5. The increase of the production of reactive oxygen intermediates both at group level and at the level of the individuals results in the intensification of the killing potential of neurophil granulocytes.

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2007 – The Psycho-physiological effects of the high OXYGEN content „KAQUN WATER” drinking cure and bath

SEMMELWEIS UNIVERSITY, Budapest, Hungary

studies 5Introduction
The results of earlier examinations suggested that the consumption of the high oxygen content kaqun water results in an increase in the oxygen saturation. On behalf of the Centrion Hungária Ltd. we have examined the change in the other physiological parameters we agreed on.

The aim of this study is to examine objectively the oxygen saturation, the reaction time, the exertion of forces, the blood pressure, the data can be derived from the ECG, the stress index and the standing stability during the continuous consumption of the high oxygen content Kaqun water and before and after a simultaneous Kaqun bath.

We can conclude that in the empirical measurements conducted we experienced favourable effects. The registered, mostly encouraging results can be the result of several factors. Among them there is the favourable effect of the high oxygen content Kaqun water by the increase in the oxygen saturation.

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2004 – Examination of the effects of high oxygen content water …

HUNGARIAN ACADEMY OF SCIENCES – INSTITUTE OF ISOTOPES, Budapest, Hungary

studies 4During our experiments we examined the encumbering effects on tumour cells of oxygenated water, that is KQN water, with mice tumour lines.

Growing of cell lines
We used 2 cell lines (H59, LLT- НН) in our experiments. The H59 is less malignant, while the LLT-НН is a Lewis Lung tumour line with a strong metastatic ability. There is a separate documentation on its deposition. We grew the tumour cells in RPМI-1640 nutritive solution, we added 5-10% of FCS (GIBCO) 0.01 М НЕРЕS and 2х10¯³ М of glutamine and we grew the tumour cells for 4-10 days in it for the purpose of the experiments.

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Studies Under Authorization

2013 – Study for the KAQUN water’s effect for humoral immune reaction

NATIONAL INSTITUTE OF CHEMICAL SAFETY –  DEPARTMENT OF MOLECULAR AND CELL BIOLOGY

Non-TUKEB

2013 – Functioning of the KAQUN water

HUNGARIAN ACADEMY OF SCIENCES – INSTITUTE OF ISOTOPES

2013 – Mouse trials for  ovarium …

UNIVERSITAS DEBRECENIENSIS